https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47832 Wed 01 Feb 2023 13:35:54 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45857 p level: 1.06 × 10^-7), that were associated with maternal smoking during pregnancy, including associated genes AHRR (cancer development), FTO (obesity), CNTNAP2 (developmental processes), CYP1A1 (detoxification), MYO1G (cell signalling) and FRMD4A (nicotine dependence). A sensitivity analysis showed a dose dependent relationship between maternal smoking and offspring methylation. These results changed little following adjustment for paternal, passive or offspring smoking and there were no CpGs identified that associated with these variables. Two of the 23 identified CpGs (cg00253568 (FTO) and cg00213123 (CYP1A1)) were associated with either TG (males and females), diastolic blood pressure (females only) or HDL-C (males only), after Bonferroni correction. Discussion: This study demonstrates a critical timing of cigarette smoke exposure over the life-course for establishing persistent changes in DNA methylation into adolescence in a dose dependent manner. There were significant associations between offspring CpG methylation and adolescent cardiovascular risk factors, namely TG, HDL-C and diastolic blood pressure. Future studies on current smoking habits and DNA methylation should consider the importance of maternal smoking during pregnancy and explore how the persistent DNA methylation effects of in utero smoke exposure increase cardiometabolic risk.]]> Tue 08 Nov 2022 08:25:41 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54919 Thu 21 Mar 2024 13:13:19 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32882 0.05) after adjustment for pretreatment values, treatment period, and treatment order. Similarly, no differences were observed between treatments for arterial stiffness measures (P > 0.05). Conclusion: Increased intake of nitrate-rich vegetables did not lower blood pressure in prehypertensive or untreated grade 1 hypertensive individuals when compared with increased intake of nitrate-poor vegetables and no increase in vegetables.]]> Thu 13 Oct 2022 17:10:46 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26366 Sat 24 Mar 2018 07:33:07 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:40086 in utero cigarette smoke exposure. Methods: We used machine learning methods to create a score reflecting exposure to maternal smoking during pregnancy. This score is based on peripheral blood measurements of DNA methylation (Illumina’s Infinium HumanMethylation450K BeadChip). The score was developed and tested in the Raine Study with data from 995 white 17-y-old participants using 10-fold cross-validation. The score was further tested and validated in independent data from the Northern Finland Birth Cohort 1986 (NFBC1986) (16-y-olds) and 1966 (NFBC1966) (31-y-olds). Further, three previously proposed DNA methylation scores were applied for comparison. The final score was developed with 204 CpGs using elastic net regression. Results: Sensitivity and specificity values for the best performing previously developed classifier (“Reese Score”) were 88% and 72% for Raine, 87% and 61% for NFBC1986 and 72% and 70% for NFBC1966, respectively; corresponding figures using the elastic net regression approach were 91% and 76% (Raine), 87% and 75% (NFBC1986), and 72% and 78% for NFBC1966. Conclusion: We have developed a DNA methylation score for exposure to maternal smoking during pregnancy, outperforming the three previously developed scores. One possible application of the current score could be for model adjustment purposes or to assess its association with distal health outcomes where part of the effect can be attributed to maternal smoking. Further, it may provide a biomarker for fetal exposure to maternal smoking.]]> Fri 22 Jul 2022 13:24:59 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39717 Fri 17 Jun 2022 17:31:05 AEST ]]>